420200

JC-1

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria.

• 別名: JC-1, 5,5ʹ,6,6ʹ-Tetrachloro-1,1ʹ,3,3ʹ-tetraethylbenzimidazolylcarbocyanine Iodide
• 実験式（ヒル表記法）: C₂₅H₂₇Cl₄IN₄
• CAS Number: 47729-63-5
• 分子量: 652.23
• MDL番号: MFCD00188475
• UNSPSCコード: 12352202
• NACRES: NA.25

特性

• 品質水準: 100
• アッセイ: ≥95% (HPLC)
• フォーム: solid
• メーカー／製品名: Calbiochem^®
• 保管条件: OK to freeze; protect from light
• 色: red-orange
• 溶解性: DMSO: 5 mg/mL
• 輸送温度: ambient
• 保管温度: −20°C
• SMILES記法: [I-].Clc1cc2[n+](c([n](c2cc1Cl)CC)\C=C\C=[c]3[n](c4c([n]3CC)cc(c(c4)Cl)Cl)CC)CC
• InChI: 1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
• InChI Key: FYNNIUVBDKICAX-UHFFFAOYSA-M

詳細

詳細

A cationic, fluorescent, carbocyanine dye that can be used as a ratiometric indicator of mitochondrial potential ΔΨ_m in cells, tissues, and isolated mitochondria. It labels mitochondria with high membrane potential orange and mitochondria with low membrane potential green. Mitochondrial depolarization is indicated by a decrease in the red/green fluorescence intensity ratio. Ideal for detection of collapse of ΔΨ_m induced by uncouplers of mitochondrial respiration. The potential-sensitive color shift is due to concentration-dependent formation of red fluorescent J-aggregates. JC-1 has also been used in a functional assay of multidrug-resistance cells and in apoptosis-related mitochondrial modifications.

生物化学的／生理学的作用

Cell permeable: no

Primary Target
Used as a ratiometric indicator of mitochondrial potential δΨm in cells, tissues, and isolated mitochondria

Product does not compete with ATP.

Reversible: no

包装

Packaged under inert gas

警告

Toxicity: Irritant (B)

再構成

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Do not reuse thawed samples.

その他情報

Mathur, A., et al. 2000. Cardiovasc. Res. 46, 126.
Gravance, C.G., et al. 2000. Theriogenology53, 1691.
Chanda, B., and Mathew, M.K. 1999. Biochim Biophys. Acta 1416, 92.
Dedov, V.N., and Roufogalis, B.D. 1999. FEBS Lett. 456, 171.
Polla, B.S., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 6458.
White, R.J., and Reynolds, I.J. 1996. J. Neurosci. 16, 5688.
Ankacrona, M., et al. 1995. Neuron 15, 961.
Reers, M., et al. 1995. Methods Enzymol. 260, 406.
Cossarizza, A., et al. 1993. Biochem. Biophys. Res. Commun. 197, 40.

法的情報

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable