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Merck

W4502

Sigma-Aldrich

Water

suitable for electrophoresis, PCR

Synonym(s):

DNase-Free Water, Not DEPC Treated, Nuclease-Free Water, Protease-Free Water, RNase-Free Water

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About This Item

Linear Formula:
H2O
CAS Number:
Molecular Weight:
18.02
Beilstein:
2050024
EC Number:
MDL number:
UNSPSC Code:
12191602
PubChem Substance ID:
NACRES:
NA.31
Technique(s):
PCR: suitable
electrophoresis: suitable
Bp:
100 °C (lit.)
Vapor pressure:
3 mmHg
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Product Name

Water, Nuclease-Free Water, for Molecular Biology

vapor density

<1 (vs air)

Quality Level

vapor pressure

3 mmHg

sterility

0.1 μm filtered

form

liquid

technique(s)

PCR: suitable
electrophoresis: suitable

refractive index

n20/D 1.34 (lit.)

pH

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

density

1.000 g/mL at 3.98 °C (lit.)

foreign activity

DNase, RNase, Protease, free

SMILES string

O

InChI

1S/H2O/h1H2

InChI key

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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Application

Water has been used-
  • in cDNA synthesis and PCR amplification
  • for the dilution of rumen DNA sample prior to qPCR assay
  • in PCR (polymerase chain reaction) and agarose gel electrophoresis
  • for the elution of RNA during RNA isolation

Features and Benefits

  • Nuclease-Free Water
  • DNase-Free, RNase-Free, Protease- Free
  • Not DEPC Treated
  • 0.1 um Filtered
  • Prepared from Double Distilled and Deionized Water
  • Suitable for use in Molecular Applications

This product is 0.1um filtered. It is designated as a Molecular Biology Reagent and is suitable for molecular biology applications. This product has been analyzed for the absence of nucleases and proteases, and has undergone bioburden analysis. It has not been treated with diethyl pyrocarbonate (DEPC).

Preparation Note

This product is prepared from double distilled and deionized water

Other Notes

Easily compare specifications for Water products with the Water specification table.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

W4502-100ML:
W4502-50ML-KC:
W4502-EW:
W4502-1L:
W4502-VAR:
W4502-1L-C:
W4502-50ML:
W4502-6X1L:
W4502-6X1L-I-PW:
W4502-BULK:
W4502PROC:
W4502-20ML-KC:
W4502-10L:4548173215167
W4502-25ML:
W4502-10ML:
W4502-6X1L-I:
W4502-10ML-KC:
W4502-1ML-KC:
W4502-5ML:
W4502-10X50ML:
W4502-20ML:
W4502-1ML:
W4502-3ML:
W4502-100ML-KC:
W4502-5ML-KC:
W4502-25ML-KC:
W4502-3ML-KC:
W4502-1.5ML:
W4502-1.5ML-KC:


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