P5936

Pectolyase from Aspergillus japonicus

suitable for plant cell culture, lyophilized powder

• Synonym(s): PEL1
• EC Number: 3.2.1.15 (BRENDA, IUBMB)
• MDL number: MFCD00131813
• UNSPSC Code: 10171502
• NACRES: NA.72

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥0.3 unit/mg solid
• greener alternative product characteristics: Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• technique(s): cell culture | plant: suitable
• application(s): agriculture
• greener alternative category: Enabling,
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.

Application

Pectolyase P5936 (Pel1) is a natural mixed pectolyase produced by the fungus Aspergillus japonicus used to digest components (endopolygalacturonate and pectin) of plant cell walls. Treatment of cell walls with pectolyase can be used to destablilize the cell walls for organelle isolation or to modify the elasticity of the cell walls.

Pectolyase from Aspergillus japonicas has been used to digest the siliques and also in immunofluorescence experiments.

Used in plant protoplast preparation to digest cell wall prior to organelle isolation.

Other Notes

Reported to contain two types of pectinase, endopolygalacturonase (EC3.2.1.15), endopectin lyase (EC4.2.2.10) and a maceration stimulating factor.

Unit Definition

One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 5.5 at 25 °C.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves