P2850

Phosphatase Inhibitor Cocktail 1

DMSO solution, mixture of inhibitors

• Synonym(s): Phosphatase Inhibitor
• EC Number: 200-664-3
• UNSPSC Code: 12352200
• NACRES: NA.54

Properties

• Product Name: Phosphatase Inhibitor Cocktail 1, DMSO solution
• form: DMSO solution
• Quality Level: 300
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.

Application

Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation. It has also been used in western blot analysis.

Biochem/physiol Actions

Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.

Quantity

Recommended usage is a 100-fold dilution.

Analysis Note

Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C