CMC0016

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

• Synonym(s): BL21 strain
• UNSPSC Code: 41106202
• NACRES: NA.85

Properties

• Product Name: BL21(DE3) Electrocompetent Cells, for protein expression
• biological source: Escherichia coli
• grade: Molecular Biology
• growth mode: adherent or suspension
• morphology: rod shaped
• technique(s): microbiological culture: suitable
• cell transformation: competent cell type: electrocompetent; transformation efficiency: ≥5 × 10⁹ cfu/μg
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 10⁹ cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Components

• BL21(DE3) electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for expression

Safety Information

• Storage Class Code: 10 - Combustible liquids