R8381

Dpn I from Diplococcus pneumoniae

Restriction Enzyme

• CAS Number: 81295-14-9
• EC Number: 3.1.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00166565
• UNSPSC Code: 12352204

Properties

• grade: Molecular Biology
• form: buffered aqueous glycerol solution
• concentration: 10,000 units/mL
• shipped in: wet ice
• storage temp.: −20°C

Description

Specificity

Recognition sequence: 5′-G^mA/TC-3′
Ligation and recutting results: After 2-10-fold Dpn I overdigestion of 1 μg pBR322 DNA substrate, results in 100% cutting, >30% of fragments can be ligated, and >90% recut.
Heat inactivation: 75 °C for 15 minutes.

Application

DpnI is a restriction endonuclease that is used in molecular biological applications to cleave the recognition sequence 5′-G^mA/TC-3′, generating DNA framents with blunt ends.

Other Notes

Supplied with 10x Restriction Enzyme Buffer SA (B7531).

Comment: Since Dpn I will completely cleave only fully methylated pBR322 DNA, cleavage of 95% or more is considered complete digestion.

Physical form

Solution in 10 mM Tris-HCl, pH 8.0, 400 mM NaCl, 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA, 50% glycerol (v/v) at 4 °C

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable