G3651

α-Glucosidase from Bacillus stearothermophilus

lyophilized powder, ≥50 units/mg protein

• Synonym(s): α-D-Glucoside glucohydrolase, Maltase
• CAS Number: 9001-42-7
• EC Number: 3.2.1.20 (BRENDA, IUBMB)
• EC Number: 232-604-7
• MDL number: MFCD00081321
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥50 units/mg protein
• greener alternative product characteristics: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• foreign activity: β-Glucosidase, α−galactosidase and β−galactosidase ≤0.1%
• greener alternative category: Enabling,
• relevant disease(s): diabetes (management of non-insulin-dependent diabetes mellitus)
• storage temp.: 2-8°C

Description

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch hydrolysis research. For more information see the article in biofiles.

Application

α-glucosidase is potential enzyme for the biosynthesis of complex carbohydrates.

Biochem/physiol Actions

α-glucosidase hydrolyzes carbohydrates by acting on 1,4-α linkages. Inhibition of α-glucosidase is a prominent target in the management of non-insulin-dependent diabetes mellitus.

Unit Definition

One unit will liberate 1.0 μmole of D-glucose from p-nitrophenyl α-D-glucoside per min at pH 6.8 at 37 °C.

Physical form

Lyophilized powder containing potassium phosphate buffer salt

Analysis Note

Activity using maltose as substrate at pH 6.0 at 25 deg C is ~2X greater than that obtained using p-nitrophenyl-α-D-glucoside as substrate at pH 6.8 at 37 °C.

Protein determined by biuret.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves