I3021

IGEPAL^® CA-630

viscous liquid

• Synonym(s): Octylphenoxy poly(ethyleneoxy)ethanol, branched
• Linear Formula: (C₂H₄O)_nC₁₄H₂₂O
• CAS Number: 9002-93-1
• UNSPSC Code: 12161900
• NACRES: NA.21

Properties

• description: non-ionic
• Quality Level: 200
• form: viscous liquid
• mol wt: ~603
• CMC: 0.08 mM (20-25°C)
• viscosity: 250-350 cP, neat(25 °C)(lit.)
• density: 1.06 g/cm³ at 25 °C (lit.)
• HLB: 13
• SMILES string: O(CCO)c1ccc(cc1)C(CC(C)(C)C)(C)C
• InChI: 1S/C16H26O2/c1-15(2,3)12-16(4,5)13-6-8-14(9-7-13)18-11-10-17/h6-9,17H,10-12H2,1-5H3
• InChI key: JYCQQPHGFMYQCF-UHFFFAOYSA-N

Description

General description

IGEPAL^® CA-630 is a nonionic, non-denaturing detergent suitable for solubilization, isolation and purification of membrane protein complexes. It is chemically indistinguishable from Nonidet P-40.

Application

IGEPAL^® CA-630 has been used:

• as a component of grinding buffer along with lithium dodecyl sulphate, tris-HCl, EDTA, NaCl, sodium deoxycholate, dithiothreitol and thiourea in RNA extraction process
• along with Tris- HCl in incubation of Tachyzoites cultured in vero cells
• as a component of nuclear extract buffer D+ containing HEPES, glycerol, KCl and EDTA for gel shift analysis
• as a component of PI buffer along with NaH₂PO₄ to wash slides in FISH (fluorescence in situ hybridization) procedure
• for solubilizing and isolation of membrane proteins

Other Notes

Nonionic, non-denaturing detergent

Quality

Chemically indistinguishable from Nonidet P-40, which is no longer commercially available.

Caution

May develop turbidity or sediment during storage. A clear liquid can be obtained on heating to 40°C.

Legal Information

IGEPAL is a registered trademark of Solvay

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 483.8 °F - closed cup
• Flash Point(C): 251 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves