NUC201

Nuclei Isolation Kit: Nuclei PURE Prep

sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)

• Sinonimo/i: Sucrose centrifugation nuclei isolation
• Codice UNSPSC: 12352207
• NACRES: NA.32

Proprietà

• impiego: sufficient for 15 nuclei preparations (~1-10×10⁷ cells or 1g of tissue per preparation)
• Livello qualitativo: 200
• Confezionamento: pkg of 1 kit
• Condizioni di stoccaggio: dry at room temperature
• applicazioni: cell analysis
• Attività estranea: nuclease and protease, free
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Applicazioni

For preparation of pure nuclei and fragile nuclei from cell lines and solid tissues.

Azioni biochim/fisiol

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

The protocol incorporates centrifugation through a dense sucrose cushion to protect nuclei and strip away cytoplasmic contaminants. The sucrose concentration that is suitable for a particular cell type is determined empirically by the user. The sucrose concentrate and sucrose cushion buffer give the user flexibility to modify the density of the sucrose cushion as appropriate. High yield has been obtained from common cell lines (Jurkat, HFN7.1, COS7, HEK293 and MDCK) and tissues (spleen and liver). These preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones, and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Solo come componenti del kit

• Nuclei PURE Lysis Buffer 180 mL

Informazioni sulla sicurezza

• Pittogrammi: GHS05,GHS09
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H318,H410
• Consigli di prudenza: P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313
• Classi di pericolo: Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable