NUC101

Nuclei Isolation Kit: Nuclei EZ Prep

sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)

• Sinonimo/i: Rapid mammalian nuclei isolation kit
• Codice UNSPSC: 12352207
• NACRES: NA.32

Proprietà

• impiego: sufficient for 25 nuclei preparations (~1-10×10⁷ cells/preparation)
• Livello qualitativo: 200
• Durata: 1 yr at 2‑8 °C
• Confezionamento: pkg of 1 kit
• applicazioni: cell analysis
• Attività estranea: nuclease and protease, free
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

The Nuclei EZ Prep Kit was developed for the rapid isolation of nuclei from mammalian cells. The kit provides a high yield of nuclei from commonly used cell types.

Applicazioni

Suitable as a source of nuclear components, to produce nuclei for in vitro apoptosis assays, and for functional studies.

Azioni biochim/fisiol

The protocol provides a high yield of nuclei from commonly used mammalian cells, including both adherent (e.g., HEK293 and COS7) and non-adherent (e.g., Jurkat and HFN7.1) cell lines and peripheral blood mononuclear cells (PBMCs). The preparations are suitable for many cell biology applications, e.g., as a source of nuclear components such as chromatin, genomic DNA, histones and nuclear RNA/RNP, produces nuclei for in vitro apoptosis assays, and functional studies such as examination of the transcriptional status of cells.

Altre note

All procedures should be carried out on ice or 2-8°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable