CMC0008

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

• UNSPSC kód: 12352200

Tulajdonságok

• Product Name: STEADY Chemically Competent Cells, for plasmid production using unstable DNA
• biológiai forrás: Escherichia coli
• grade: Molecular Biology
• Forma: buffered aqueous solution
• növekedési mód: adherent or suspension
• morfológia: rod shaped
• technika/technikák: microbiological culture: suitable
• sejttranszformáció: competent cell type: chemically competent; transformation efficiency: >1 x 10⁸ cfu/μg
• kiszállítva: dry ice
• tárolási hőmérséklet: −70°C

Leírás

Általános leírás

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Tulajdonságok és előnyök

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chemi) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

Komponensek

• STEADY chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids