17783

Calcein-AM

BioReagent, suitable for fluorescence, ≥90% (HPLC)

• Szinonimák: Calcein acetoxymethyl ester, Calcein O,O′-diacetate tetrakis(acetoxymethyl) ester
• Tapasztalati képlet (Hill-képlet): C₄₆H₄₆N₂O₂₃
• CAS Number: 148504-34-1
• Molekulatömeg: 994.86
• MDL-szám: MFCD05861516
• UNSPSC kód: 12171500
• PubChem Substance ID: 57647670
• NACRES: NA.32

Tulajdonságok

• termékcsalád: BioReagent
• Minőségi szint: 100
• Teszt: ≥90% (HPLC)
• fluoreszcencia: λ_ex 496 nm; λ_em 516 nm in 0.1 M Tris pH 8.0, esterase; Ca²⁺
• alkalmasság: suitable for fluorescence
• tárolási hőmérséklet: −20°C
• SMILES string: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45
• InChI: 1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
• Nemzetközi kémiai azonosító kulcs: XKFSBWQWNMZWFA-UHFFFAOYSA-N

Leírás

Általános leírás

Liposomes contain a self-quenching fluorescent dye called calcein. It is a fluorescent dye with excitation and emission wavelengths of 495/515 nm, respectively. It self-quenches at concentrations above 70mM and is commonly used as an indicator of lipid vesicle leakage. The leakage rate is calculated on the basis of the fluorescence increment as the entrapped calcein is leaked out of the liposomal compartment. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

Alkalmazás

Calcein-AM has been used in flow cytometry.

Non-fluorescent cell permeable derivative of calcein, becomes fluorescent on hydrolysis. Used as a neutral substrate for multidrug resistance protein, MRP; employed in tumor research.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)