SEQR

SeqPlex RNA Amplification Kit

For use with high throughput sequencing technologies

• Sinónimos: WGA kit, Whole transcriptome amplification
• Código UNSPSC: 41121800
• NACRES: NA.55

Propiedades

• Nivel de calidad: 200
• técnicas: whole genome amplification: suitable
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

The SeqPlex^™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.

Aplicación

SeqPlex^™ RNA Amplification Kit has been used to amplify poly(A)-selected RNA.

Características y beneficios

• Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
• Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
• No need to fragment DNA before sequencing.
• Amplifies ds-cDNA in 8 hours or less.
• Compatible with all next generation sequencing platforms except Pacific Bioscience.

Otras notas

SEQR-500RXN is manufactured on-demand. Contact us for more information.

Información legal

SeqPlex is a trademark of Sigma-Aldrich Co. LLC

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable