A8011

Antifoam C Emulsion

aqueous-silicone emulsion

• Synonym(s): Antifoaming Agent Emulsion
• UNSPSC Code: 12161900
• NACRES: NA.25

Properties

• biological source: synthetic
• Quality Level: 200
• form: emulsion
• contains: emulsifier
• technique(s): cell culture | hybridoma: suitable; microbiological culture: suitable
• mp: 0 °C (32 °F)

Description

General description

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

Application

Antifoam C emulsion has been used:

• as a supplement in RO-H₂O and a chemically defined culture medium to determine volumetric mass transfer coefficient (k_La) of mammalian cell culture
• as a component of feed medium,
• as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
• as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

Features and Benefits

• Minimizes compatibility issues with biological systems
• Highly effective defoamer at low concentrations
• Highly effective and versatile silicone defoamer

Other Notes

A 30% aqueous emulsion of polydimethylsiloxane concentrate.

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Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H319,H335
• Precautionary Statements: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves