P3563

Phosphate Buffered Saline

powder, pH 7.4, with 0.05% Tween^® 20

• Synonym(s): 1X PBS + 0.05% TWEEN^® 20, 1X PBS + 0.05% TWEEN^® 20, PBS-T, PBST
• UNSPSC Code: 41105319
• eCl@ss: 32129211
• NACRES: NA.25

Properties

• Product Name: Phosphate Buffered Saline with 0.05% TWEEN^® 20, pH 7.4,
• form: powder
• Quality Level: 200
• solubility: water: soluble, clear, colorless (1 pouch dissolved in 1L)
• application(s): cell analysis; diagnostic assay manufacturing

Description

General description

Phosphate buffered saline with TWEEN^® 20, or PBS-T, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN 20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBS-T buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

Application

Phosphate buffered saline has been used in:

• western blotting
• enzyme-linked immunosorbent assay (ELISA)
• telomere-FISH (Tel-FISH) and telomere/immunostaining-FISH (Teli-Fish)
• cell isolation

Packaging

Foil pouches

Reconstitution

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

Other Notes

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Legal Information

TWEEN is a registered trademark of Croda International PLC

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves