WTA1

TransPlex^® Whole Transcriptome Amplification Kit

DNA polymerase separate.

• Sinónimos: Transcriptome Amplification Kit
• Código UNSPSC: 41121800
• NACRES: NA.55

Propiedades

• Nivel de calidad: 200
• técnicas: whole genome amplification: suitable
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

TransPlex^®, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3′-bias. Microgram quantities of amplification product generated from tissue, cultured cells, formalin-fixed samples, or serum are suitable for downstream applications such as qPCR and microarray analyses.

Aplicación

Suitable for use with downstream applications including:

• qPCR
• microarray analysis
• cloning

TransPlex^® Whole Transcriptome Amplification Kit has been used to synthesize double-stranded cDNA. It has also been used in the amplification of cDNA.

Características y beneficios

• Amplification of total RNA in less than 4 hours with less than 30 minutes of "hands-on" time
• Only 5 ng of starting material required to produce a highly representative library from total RNA
• Microgram quantities of amplification product generated from intact RNA from tissue, cultured cells, serum, or degraded RNA from formalin-fixed paraffin-embedded samples

Principio

The WTA process involves two steps. In the first step, sample RNA is reverse transcribed with non-self-complementary primers composed of a quasi-random 3′ end and a universal 5′ end. As polymerization proceeds, displaced single strands serve as new templates for primer annealing and extension. The resultant OmniPlex cDNA library, comprised of random, overlapping 100 - 1000 base fragments flanked by universal end sequence, is then amplified by PCR with the universal primer to produce WTA product.

Información legal

TransPlex is a registered trademark of Rubicon Genomics, Inc.

Los componentes del kit también están disponibles por separado

• W4502 Water, Nuclease-Free Water, for Molecular Biology
• D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H319
• Consejos de prudencia: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Eye Irrit. 2
• Código de clase de almacenamiento: 10 - Combustible liquids
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable