T3038

Trizma^® hydrochloride solution

1 M, BioReagent

• Synonym(s): Tris hydrochloride solution
• MDL number: MFCD00012590
• UNSPSC Code: 12161700
• PubChem Substance ID: 24900102
• NACRES: NA.25

Properties

• grade: Molecular Biology
• Quality Level: 200
• sterility: 0.2 μm filtered
• product line: BioReagent
• form: solution
• concentration: 1 M
• impurities: DNase, RNase, NICKase and protease, none detected
• pH: 8.0
• application(s): agriculture
• SMILES string: Cl.NC(CO)(CO)CO
• InChI: 1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
• InChI key: QKNYBSVHEMOAJP-UHFFFAOYSA-N

Description

General description

Trizma^® hydrochloride solution is a useful biological buffer.

Application

The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per degree C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Trizma^® hydrochloride solution may be used in the following studies:

• As buffer for the 2-D electrophoresis of rat fibroblast cell.
• As buffer for the rapid isolation of high molecular weight plant DNA (50,000 base pairs or more in length).
• Selective immunoprecipitation of biotin-labeled DNA with antibiotin IgG and Staphylococcus sp.

Other Notes

Prepared with pH-adjusted Biotechnology Performance Certified Trizma Base in 18 megohm water and 0.2 μm filtered.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves