B5525

Bromophenol Blue sodium salt

• Synonym(s): 3′,3′′,5′,5′′-Tetrabromophenolsulfophthalein sodium salt
• Empirical Formula (Hill Notation): C₁₉H₉Br₄NaO₅S
• CAS Number: 34725-61-6
• Molecular Weight: 691.94
• Beilstein: 6995701
• EC Number: 252-170-2
• MDL number: MFCD00013793
• UNSPSC Code: 12171500
• PubChem Substance ID: 57653941
• NACRES: NA.52

Properties

• form: powder
• Quality Level: 200
• technique(s): electrophoresis: suitable
• solubility: water: soluble
• suitability: suitable for nucleic acid electrophoresis
• storage temp.: room temp
• SMILES string: [Na+].Oc1c(Br)cc(cc1Br)C(=C2/C=C(Br)C(=O)C(Br)=C2)\c3ccccc3S([O-])(=O)=O
• InChI: 1S/C19H10Br4O5S.Na/c20-12-5-9(6-13(21)18(12)24)17(10-7-14(22)19(25)15(23)8-10)11-3-1-2-4-16(11)29(26,27)28;/h1-8,24H,(H,26,27,28);/q;+1/p-1
• InChI key: TWKDJMRHSLWRFI-UHFFFAOYSA-M

Description

General description

Bromophenol Blue is a pH indicator, and a dye appearing as a strong blue color. It is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. Bromophenol blue has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.

Application

A tracking dye for nucleic acid or protein electrophoresis in agarose or polyacrylamide gels.

Bromophenol blue sodium salt has been used in the preparation of protein samples for western blotting analysis. It has also been used in Drosphila fly food to aid visualization of gut for dissection.

Reconstitution

A 6x Gel Loading Buffer can be made using standard recipes, including 0.015% bromophenol blue.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves