HPLC Analysis of PAHs in Water on SUPELCOSIL™ LC-PAH after SPME using 100 μm PDMS Fiber
Materials
SPME fiber
Product No.
Description
Pricing
SPME Fiber Assembly
100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, plain red hubCONDITIONS
sample/matrix
5 μL PAH mix (Cat. No. 4-8743) in 5 mL water
SPME fiber
polydimethylsiloxane, 100 μm (57301)
extraction
immersion, 30 min (rapid stirring)
desorption process
static, 200 μL acetonitrile:water, 40:60, 2 min
column
SUPELCOSIL LC-PAH, 15 cm × 4.6 mm I.D., 5 μm particles (58318)
gradient
0 min - 50% A; 5 min - 50% A; 30 min - 100% A; 45 min - 100% A
flow rate
0-2 min: 0.2 mL/min 2-45 min: 1.0 mL/min
mobile phase
[A] acetonitrile:[B] water
detector
UV, 254 nm
Description
Legal Information
SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC