Product Name
Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture
biological source
mouse
Quality Level
recombinant
expressed in NSO cells
sterility
sterile
Assay
>95% (SDS-PAGE)
form
lyophilized powder
mol wt
30 kDa by SDS-PAGE (reducing)
65 kDa by SDS-PAGE (reducing) (doublet)
packaging
pkg of 50 μg
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
mouse ... Spp1(20750)
Application
Osteopontin from mouse has been used:
- to study its role in a mouse model of preterm brain injury
- as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
- to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels
Biochem/physiol Actions
Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to mediate 293 cell adhesion.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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