{"metaDataObj":{"isNoIndex":false,"isNoFollow":false,"keywords":["Small molecule HPLC","Protocol","Clinical and Forensics Testing"],"title":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5","seoSchemas":[{"@context":"https://schema.org","@type":"BreadcrumbList","itemListElement":[{"@type":"ListItem","position":1,"name":"Small Molecule HPLC","item":"%%HOMEPAGE_URL%%/AR/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc"},{"@type":"ListItem","position":2,"name":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis^® Express OH5"}]}],"description":"Separation of Pyridoxine Hydrochloride (B6), analytical standard; Thiamine Hydrochloride (B1), analytical standard","pageType":"Protocol","ogImageMetaInfo":[{"ogTitle":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5","ogType":"website","ogImage":"https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/social-media/vibrant-m/vibrant-m.jpg","ogUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6","ogDescription":"Separation of Pyridoxine Hydrochloride (B6), analytical standard; Thiamine Hydrochloride (B1), analytical standard"}]},"designPath":"/libs/settings/wcm/designs/default","cssClassNames":"page basicpage","title":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5","templateName":"technical-article---protocol-page-template","lastModifiedDate":1645950785732,"language":"en",":itemsOrder":["root"],":items":{"root":{"columnClassNames":{"rightrailresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","footer":"aem-GridColumn aem-GridColumn--default--12","utilityresponsivecontainer":"aem-GridColumn aem-GridColumn--default--3 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","bodyresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","topresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9 aem-GridColumn--offset--default--0 aem-GridColumn--default--none"},"gridClassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columnCount":12,"allowedComponents":{"applicable":false,"components":[{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/aboutus","title":"About Us Text"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/accordion","title":"Accordion"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/audio","title":"Audio"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/author","title":"Author"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/breadcrumb","title":"Breadcrumbs - Page Path"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/button","title":"Button"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/card","title":"Card"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/carousel","title":"Carousel"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/categorysearchresults","title":"Category Search Results"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/cftext","title":"CF Text"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/contact","title":"Contact Component"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/container","title":"Container component"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/dealerlicense","title":"Dealer License"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/dynamicproducttable","title":"Dynamic Product Table"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/event-sidebar","title":"Event Sidebar"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/eventlisting","title":"Event Listing"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/experiencefragment","title":"Experience Fragment"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/featuretiles","title":"Feature Tiles"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/footer","title":"Footer"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/formAssembly","title":"Form Assembly"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/gatedbutton","title":"Gated Button"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/gatedcontent","title":"Gated Content"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/globalfooter","title":"Global Footer"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/header","title":"Header"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/herocomponent","title":"Hero Component"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/workflows/horizontal","title":"Workflow Horizontal"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/horizontaltab","title":"Tabbed Horizontal Navigation"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/image","title":"Image"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/leftnavigation","title":"Navigation Model"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/locationmap","title":"Map"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/pressreleasegrid","title":"Related Press releases"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/pressreleaselisting","title":"Press release listing"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/presstext","title":"Region Text"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/primarycontent","title":"Primary Content"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/printsocialsharing","title":"Print Social sharing"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/productRecommendationCarousel","title":"Product Recommendation Carousel"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/promobanner","title":"Promo Banner"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/rawhtml","title":"Raw HTML"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/recommendedSearch","title":"Recommended Search Term"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/reference","title":"Reference"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedContentCarousel","title":"Related Content Carousel"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedcontentgrid","title":"Related Content Grid"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedcontentlinks","title":"Related Content Links"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedproductresources","title":"Related Product Resources"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedproducts","title":"Related Products Carousel"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedproductsrightrail","title":"Related Products Right Rail"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/relatedwebinar","title":"Related Webinar"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/sectiondivider","title":"Section Divider"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/sitecontentsearch","title":"Site Content Search"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/sitemap","title":"Site Map"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/speakerpage","title":"Speaker Page"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/speakers-list","title":"Speakers List"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/table","title":"Static Table"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/tagbasedbreadcrumb","title":"Breadcrumbs - Tag Based"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/targetcontainer","title":"Target Container component"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/targetescapehatch","title":"Target Escape Hatch"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/text","title":"Text"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/textbanner","title":"Text Banner"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/title","title":"Title"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/topPagesCarousel","title":"Top Pages Carousel"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/topnavigation","title":"Top Navigation"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/upcomingeventsgrid","title":"Upcoming Events"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/workflows/vertical","title":"Workflow Vertical"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/video","title":"Video"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/webinarlisting","title":"Webinar listing"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/webinarsidebar","title":"Webinar Sidebar"},{"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/cms-commons/components/content/worldwideofficelisting","title":"Worldwide Office Listing"}]},":itemsOrder":["topresponsivecontainer","utilityresponsivecontainer","bodyresponsivecontainer","rightrailresponsivecontainer","footer"],":items":{"topresponsivecontainer":{"columnClassNames":{"tagbasedbreadcrumb":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9","title":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1645950785350,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/topresponsivecontainer/tagbasedbreadcrumb","name":"tagbasedbreadcrumb"},{"url":null,"title":null,"description":null,"lastModified":1645950785359,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/topresponsivecontainer/title","name":"title"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["tagbasedbreadcrumb","title"],":items":{"tagbasedbreadcrumb":{"items":[{"url":"/AR/en","title":"Home","titleEng":"Home","active":false,"nonClickable":false},{"url":"/AR/en/applications/analytical-chemistry/liquid-chromatography/small-molecule-hplc","title":"Small Molecule HPLC","titleEng":"Small Molecule HPLC","active":false,"nonClickable":false},{"url":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6","title":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis^® Express OH5","titleEng":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis^® Express OH5","active":true,"nonClickable":true}],":type":"cms-commons/components/content/tagbasedbreadcrumb"},"title":{"anchorId":null,"openNewTab":false,"linkURL":null,"text":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis^® Express OH5","type":"h1",":type":"cms-commons/components/content/title"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"utilityresponsivecontainer":{"columnClassNames":{"cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"cms-container--top","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"bodyresponsivecontainer":{"columnClassNames":{"reference":"aem-GridColumn aem-GridColumn--default--9","bottomcontainer":"aem-GridColumn aem-GridColumn--default--9","dynamicproducttable":"aem-GridColumn aem-GridColumn--default--9","middleresponsivecontainer":"aem-GridColumn aem-GridColumn--default--9","topcontainer":"aem-GridColumn aem-GridColumn--default--9","cq:responsive":"aem-GridColumn aem-GridColumn--default--9"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--9","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1645950785457,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/topcontainer","name":"topcontainer"},{"url":null,"title":null,"description":null,"lastModified":1645950785543,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/dynamicproducttable","name":"dynamicproducttable"},{"url":null,"title":null,"description":null,"lastModified":1645950785551,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/middleresponsivecontainer","name":"middleresponsivecontainer"},{"url":null,"title":null,"description":null,"lastModified":1645950785558,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/reference","name":"reference"},{"url":null,"title":null,"description":null,"lastModified":1645950785565,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/bottomcontainer","name":"bottomcontainer"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["topcontainer","dynamicproducttable","middleresponsivecontainer","reference","bottomcontainer"],":items":{"topcontainer":{"columnClassNames":{"image":"aem-GridColumn aem-GridColumn--default--7 aem-GridColumn--offset--default--0 aem-GridColumn--default--none","table_1183631280":"aem-GridColumn aem-GridColumn--default--12","table":"aem-GridColumn aem-GridColumn--default--12"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1645950785492,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/topcontainer/image","name":"image"},{"url":null,"title":null,"description":null,"lastModified":1645950785522,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/topcontainer/table","name":"table"},{"url":null,"title":null,"description":null,"lastModified":1645950785532,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/bodyresponsivecontainer/topcontainer/table_1183631280","name":"table_1183631280"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["image","table","table_1183631280"],":items":{"image":{"anchorId":null,"src":"/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/articles/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamin-b1-b6/lc-ms-ms-analysis-of-vitamin-b1-b6.jpg","alt":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5","externalUrl":false,"title":"LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5","uuid":"c76d39b8-2948-411c-8a13-103c639202b0","widths":[],"link":"","caption":"","displayPopupTitle":true,":type":"cms-commons/components/content/image"},"table":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Conditions","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

column	Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 µm particles (53757-U)
column temp.	30 °C
mobile phase	[A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85)
flow rate	0.2 mL/min
pressure	1150 psi (79 bar)
sample	10 ng/mL in mobile phase
injection	1 µL
detector	MS, ESI(+), MRM mode

\r\n",":type":"cms-commons/components/content/table"},"table_1183631280":{"anchorId":null,"enableSort":false,"verticalTable":false,"headline":"Description","title":null,"caption":null,"table":"\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n\r\n

Analysis Note	The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis^® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation.
Categories	Analytical Chromatography, Immunosuppressants
Featured Industry	Vitamins, Nutraceuticals, and Natural Products
suitability	application for HPLC

\r\n",":type":"cms-commons/components/content/table"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"dynamicproducttable":{"anchorId":null,"productsArray":["5.33001 Supelco (MM)","1.00029 Supelco (MM)","73594 Supelco (SIAL)","70221 Supelco (SIAL)","53757-U Supelco (SUPELCO)","1.11727 Supelco (MM)","5.33002 Supelco (MM)","1.06007 Supelco (MM)","1.06035 Supelco (MM)","5.43841 Supelco (MM)","PHR1036 Supelco (SIAL)","47862 Supelco (SUPELCO)","PHR1037 Supelco (SIAL)","V-014 Supelco (CERILLIAN)","47858 Supelco (SUPELCO)","69372 Millipore (SIAL)","1.15333 Supelco (MM)"],"isShowImage":false,"maxRows":20,"products":[{"productKey":"533001","brandKey":"MM"},{"productKey":"100029","brandKey":"MM"},{"productKey":"73594","brandKey":"SIAL"},{"productKey":"70221","brandKey":"SIAL"},{"productKey":"53757U","brandKey":"SUPELCO"},{"productKey":"111727","brandKey":"MM"},{"productKey":"533002","brandKey":"MM"},{"productKey":"106007","brandKey":"MM"},{"productKey":"106035","brandKey":"MM"},{"productKey":"543841","brandKey":"MM"},{"productKey":"PHR1036","brandKey":"SIAL"},{"productKey":"47862","brandKey":"SUPELCO"},{"productKey":"PHR1037","brandKey":"SIAL"},{"productKey":"V014","brandKey":"CERILLIAN"},{"productKey":"47858","brandKey":"SUPELCO"},{"productKey":"69372","brandKey":"SIAL"},{"productKey":"115333","brandKey":"MM"}],"tableTitle":"Materials",":type":"cms-commons/components/content/dynamicproducttable"},"middleresponsivecontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"reference":{"headline":null,"anchorId":null,"startNumber":null,"references":null,":type":"cms-commons/components/content/reference"},"bottomcontainer":{"columnClassNames":{},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--12 aem-Grid--default--12","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":[],":items":{},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"rightrailresponsivecontainer":{"columnClassNames":{"relatedcontentlinks__6877194":"aem-GridColumn aem-GridColumn--default--3","relatedcontentlinks_":"aem-GridColumn aem-GridColumn--default--3","cq:responsive":"aem-GridColumn aem-GridColumn--default--3"},"cssClassName":"","paddingLeftRight":"0px","gridclassNames":"aem-Grid aem-Grid--default--3","columns":12,"hasFeatureCard":false,"halfGatedContent":false,"items":[{"url":null,"title":null,"description":null,"lastModified":1645950785629,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks_","name":"relatedcontentlinks_"},{"url":null,"title":null,"description":null,"lastModified":1645950785638,"path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6/jcr:content/root/rightrailresponsivecontainer/relatedcontentlinks__6877194","name":"relatedcontentlinks__6877194"}],"allowedComponents":{"applicable":false,"components":[]},":itemsOrder":["relatedcontentlinks_","relatedcontentlinks__6877194"],":items":{"relatedcontentlinks_":{"contentType":"sigma-aldrich:content-type/page-type/technical-article","linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"/AR/en/search/facet-search?focus=sitecontent&term=facet-search&facet=facet_content_type%3ATechnical+Article&facet=facet_applications%3ASmall+molecule+HPLC","title":"Related Articles","tags":["sigma-aldrich:applications/analytical-chemistry/small-molecule-hplc"],"ctaTitle":null,"categories":[{"categoryTitle":"HPLC Separation of Ginsenosides from American Ginseng","categoryUrl":"/AR/en/technical-documents/protocol/analytical-chemistry/small-molecule-hplc/separation-ginsenosides","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Determination of Formaldehyde and Acetaldehyde in Air Using DNPH Cartridges and Automated On-Line Desorption Followed by HPLC","categoryUrl":"/AR/en/technical-documents/protocol/environmental-testing-and-industrial-hygiene/air-testing/aldehydes-air-dnph","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Cannabinoid Certified Reference Materials (CRMs) for an Improved Testing Accuracy and Traceability","categoryUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/cannabinoid-certified-reference-materials-improved-testing-accuracy-traceability","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Solution with 17 Amino Acids as TraceCERT® Certified Reference Material","categoryUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/certified-amino-acid-mix-solution","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Isotopically Labeled Steroid Standards","categoryUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/labeled-steroids","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Reference Standards for Analyzing Polyphenol Catechins","categoryUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/reference-standards","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Certified Reference Materials: Synthesis and Characterization of Cocaine N-oxide HCl","categoryUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/synthesis-and-characterization-of-cocaine-n-oxide-hcl","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Synthesis & Characterization of ±-threo-Ritalinic Acid-D₁₀ Hydrochloride","categoryUrl":"/AR/en/technical-documents/technical-article/analytical-chemistry/calibration-qualification-and-validation/synthesis-and-characterization-of-threo-ritalinic-acid-d10-hydro","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"},"relatedcontentlinks__6877194":{"linksNumber":8,"anchorId":null,"rightRail":false,"ctaLink":"","title":"Related Product Categories","tags":null,"ctaTitle":null,"categories":[{"categoryTitle":"Vitamin Standards","categoryUrl":"/AR/en/products/analytical-chemistry/reference-materials/vitamin-standards","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Pharmacopeia & Metrological Institute Standards","categoryUrl":"/AR/en/products/analytical-chemistry/reference-materials/pharmacopeia-and-metrological-institutes-standards","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Food & Cosmetic Component Standards","categoryUrl":"/AR/en/products/analytical-chemistry/reference-materials/food-and-cosmetic-component-standards","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"Pharmaceuticals, Illicit Drugs & Diagnostic Biomarkers","categoryUrl":"/AR/en/products/analytical-chemistry/reference-materials/pharmaceuticals-illicit-drugs-and-diagnostic-biomarkers","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false},{"categoryTitle":"HPLC Columns","categoryUrl":"/AR/en/products/analytical-chemistry/analytical-chromatography/hplc-columns","thumbnail":"https://wwwpws.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/images/default/default-link-image/default-link-image.png","externalUrl":false}],":type":"cms-commons/components/content/relatedcontentlinks"}},"authenticatedUserBGColor":"","unauthenticatedUserBGColor":"",":type":"cms-commons/components/content/container"},"footer":{"richText":false,":type":"cms-commons/components/content/footer"}},":type":"wcm/foundation/components/responsivegrid"}},":hierarchyType":"page",":type":"cms-commons/components/structure/page",":path":"/AR/en/technical-documents/technical-article/analytical-chemistry/small-molecule-hplc/lc-ms-ms-analysis-of-vitamins-b1-and-b6"}