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Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Dextran sulfates are supplied as the sodium salt forms, making them soluble and stable in water. Dextran sulfate contains approximately 17% sulfur which is equivalent to approximately 2.3 sulfate groups per glucosyl residue.

Neuraminidase (Sialidase) Protocol

Puriﬁcation using MBPTrap™ HP Columns

This page shows how to purify MBP-tagged proteins using MBPTrap HP columns from GE Healthcare.

Whole Gene Amplification Formalinfixed Paraffin embedded Tissues

Archived formalin-fixed and paraffin-embedded (FFPE) tissue collections represent invaluable resources for studying pathogenesis of cancer and a variety of other diseases.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL

Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.

HPLC Analysis of Various Cationic Polymers on TSKgel® PWXL-CP Columns

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan

KiCqStart™ One-Step Probe RT-qPCR ReadyMix™

KiCqStart One-Step Probe RT-qPCR ReadyMix is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan 5'-hydrolysis probes on real-time PCR systems.

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Typical Procedures for Polymerizing via RAFT

We presents an article featuring procedures that describe polymerization of methyl methacrylate and vinyl acetate homopolymers and a block copolymer as performed by researchers at CSIRO.

Buffer Reference Center

Choose a buffer based on your pH requirements as well as the pKa, a measure of acid strength that accounts for pH, concentration, and temperature.

ALiCE®Cell-Free Protein Synthesis System Protocol

Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.

Enzymatic Assay and ATP Sensitivity Test of Luciferase

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Enzymatic Assay of Pectinase

Lipid Transfection Optimization Protocol

Chromatin Isolation by RNA Purification (ChIRP) Protocol

Protocol for Chromatin Isolation by RNA Purification (ChIRP) Protocol

Duolink® PLA Probemaker Guide

Guide to create custom PLA probes for immunofluorescent or brightfield detection

Protocols for Membrane Scaffold Proteins and Nanodisc Formation

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

RNAstable® Protocol

Biomatrica nucleic acid stability products for RNA preservation.

Counting Yeast Cells for Brewing and Wine Industries Can Be Facilitated by the Scepter™ 2.0 Handheld Automated Cell Counter

The Scepter used in an application to count yeast cells.

Enzymatic Assay of Aprotinin

Oligonucleotide Melting Temperature

Calculate the melting temperature (Tm) of oligonucleotides using theoretical method like the nearest neighbor or the basic method. The DNA Tm is the temperature at which 50% of the oligonucleotide is duplexed with its perfect complement and 50% is free

Coating Tissue Culture Plates with Vitronectin

Vitronectin is used to coat culture vessels for cells attachment.

Plant/Fungi DNA Isolation Kit Protocol

The Plant/Fungi DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of plant and fungi species.

Analytical Method: Phosphorus (total) in dairy products

Phosphorus (total) in dairy products - Photometric determination using the Phosphorus molybdenum blue method subsequent to acid mineralisation

Webinar: High-precision PCR using ThermaGenix Reagents

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

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