- Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Gene (2014-02-04)
Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya
PMID24487090
ABSTRACT
Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.
MATERIALS
Product Number
Brand
Product Description
Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%
Nickel, wire reel, 50m, diameter 0.25mm, as drawn, 99%
Nickel, wire reel, 250m, diameter 0.125mm, as drawn, 99%
Nickel, wire reel, 200m, diameter 0.125mm, hard, 99.98%
Nickel, tube, 200mm, outside diameter 1.3mm, inside diameter 1.1mm, wall thickness 0.1mm, hard, 99.5%
Nickel, foil, 25mm disks, thickness 0.05mm, as rolled, 99.98%
Nickel, foil, 1m coil, thickness 0.03mm, 99.9%
Nickel, tube, 1000mm, outside diameter 3.25mm, inside diameter 3.05mm, wall thickness 0.1mm, hard, 99.5%
Nickel, tube, 1000mm, outside diameter 0.762mm, inside diameter 0.204mm, wall thickness 0.279mm, hard, 99.5%
Nickel, foil, 15mm disks, thickness 0.5mm, annealed, 99.98%
Nickel, tube, 200mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, hard, 99.5%
Nickel, wire reel, 5m, diameter 0.025mm, as drawn, 99.6+%
Nickel, wire reel, 500m, diameter 0.4mm, as drawn, 99%
Nickel, wire reel, 20m, diameter 0.125mm, hard, 99.98%
Nickel, tube, 500mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%
Nickel, tube, 1000mm, outside diameter 1.5mm, inside diameter 1.4mm, wall thickness 0.05mm, hard, 99.5%
Nickel, foil, 1m coil, thickness 0.0125mm, 99.9%
Nickel, wire reel, 1m, diameter 1.0mm, hard, 99.98%
Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.5mm, wall thickness 0.15mm, as drawn, 99.5%
Nickel, foil, 25mm disks, thickness 0.03mm, 99.9%
Nickel, foil, 10m coil, thickness 0.3mm, coil width 1mm, annealed, 99%
Nickel, microfoil, disks, 25mm, thinness 0.1μm, specific density 93.4μg/cm2, permanent mylar 3.5μm support, 99.99+%
Nickel, foil, 150x150mm, thickness 0.25mm, annealed, 99.98%
Nickel, wire reel, 100m, diameter 0.5mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.0125mm, 99.99+%
Nickel, wire reel, 0.2m, diameter 1.0mm, as drawn, 99.99+%
Nickel, foil, 0.5m coil, thickness 0.25mm, annealed, 99%
Nickel, wire reel, 1m, diameter 0.5mm, as drawn, 99.99+%