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  • Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Quantitative analysis for organic acids in biological samples: batch isolation followed by gas chromatographic-mass spectrometric analysis.

Clinical chemistry (1989-04-01)
G Hoffmann, S Aramaki, E Blum-Hoffmann, W L Nyhan, L Sweetman
ABSTRACT

This new method for qualitative and quantitative determination of organic acids, aldehydes, and ketones in biological samples is effective for use with urine, plasma, and amniotic fluid, and it requires no deproteinization. Isolation by batch-wise liquid partition chromatography on silicic acid follows formation of the O-(2,3,4,5,6-pentafluorobenzyl)oximes of oxoacids, aldehydes, and ketones. The total organic acid content of the sample provides a rapid screening test for metabolic abnormality. A wide-bore, bonded-phase capillary column was used for quantitative gas chromatographic-mass spectrometric analysis, followed by automated identification and quantification. Analytical recoveries were quantitative for a wide variety of metabolites. Gas-chromatographic retention indices, discriminating ions, and control ranges in amniotic fluid, plasma, and urine of adult subjects were determined for 61 biologically important compounds.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
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L-(−)-Malic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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4-Hydroxybenzoic acid, ReagentPlus®, ≥99%
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4-Hydroxybenzoic acid, ReagentPlus®, 99%
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Adipic acid, 99%
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Melting point standard 121-123°C, analytical standard
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Adipic acid, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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L-(−)-Malic acid, ≥95% (titration)
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L-(−)-Malic acid, ReagentPlus®, ≥99%
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L-(−)-Malic acid, 97%, optical purity ee: 99% (GLC)
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L-(+)-Lactic acid, 80%
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Succinic acid, BioXtra, BioRenewable, ≥99.0%
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Succinic acid, puriss. p.a., ACS reagent, ≥99.5% (T)
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Succinic acid, purum p.a., ≥99.0% (T)
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Stearic acid, ≥95%, FCC, FG
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Suberic acid, 98%
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Stearic acid, reagent grade, 95%
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Oleic acid, meets analytical specification of Ph, Eur., 65.0-88.0% (GC)
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Oleic acid, natural, FCC
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Fumaric acid, FCC, FG
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Fumaric acid, ≥99.0% (T)
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L-(+)-Tartaric acid, ACS reagent, ≥99.5%
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Benzoic acid, Standard for quantitative NMR, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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