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About This Item
Linear Formula:
H2NCOCH2CH2CH(NH2)CO2H
CAS Number:
Molecular Weight:
146.14
Beilstein:
1723797
EC Number:
MDL number:
UNSPSC Code:
12352209
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75
Quality Level
sterility
γ-irradiated
product line
BioXtra
form
powder
packaging
vial of 0.292 g
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
color
white to faint yellow
mp
185 °C (dec.) (lit.)
solubility
H2O: 10 mL/vial, clear to slightly hazy
SMILES string
N[C@@H](CCC(N)=O)C(O)=O
InChI
1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key
ZDXPYRJPNDTMRX-VKHMYHEASA-N
Gene Information
rat ... Ggt1(116568)
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Application
L-Glutamine has been used as a media supplement:
- for cochlear organotypic culturing
- in Iscove′s modified Dulbecco′s medium for HeLa cells culturing
- in Dulbecco′s modified Eagle′s medium for primary hepatocyte cell culturing
Biochem/physiol Actions
L-Glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-Glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Glutamine mediates protein turnover via cellular mTOR (mammalian target of rapamycin) signalling. It is also known to be associated with the inhibition of apoptosis.
L-glutamine is an essential amino acid that is a crucial component of culture media, and serves as a major energy source for cells in culture. Optimal cell performance usually requires supplementation of the medium with L-glutamine prior to use.
Packaging
50 mL amber serum vial with rubber stopper.
Suitability
Qualified for use in cell culture applications, especially serum-free cell culture.
Reconstitution
Prepares a 200 mM solution when reconstituted to 10 mL with sterile water.
Other Notes
The dipeptide L-Ala-LGln is frequently used in modern serum-free media formulations because it provides a more stable form of glutamine in vitro.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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