B6429
Casein Blocking Buffer 10x
for Northern and Southern blotting, solution
Synonym(s):
10x Blocking Buffer
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.54
Recommended Products
grade
Molecular Biology
Quality Level
form
solution
technique(s)
Southern blotting: suitable
storage temp.
room temp
Related Categories
General description
Casein is a phosphoprotein found in milk. It is classified into four subtypes, including αS1-, αS2-, β-, and κ-caseins.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Sigma’s 10x Blocking Buffer is a casein-based blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes.
Application
Casein Blocking Buffer 10x has been used in:
- western blotting
- enzyme linked immunosorbent assay (ELISA)
- immunohistochemistry
Features and Benefits
- Better blocking performance than dried milk or BSA
- Easy-to-use concentrate
- Compatible with neutral and positively-charged membranes
- Compatible with chemiluminescent and colorimetric detection assays
Preparation Note
Dilute 10x Blocking Buffer with molecular biology grade water before use.
recommended
Product No.
Description
Pricing
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Protocols
Membrane-based blotting applications that employ enzyme conjugates to generate colorimetric or chemiluminescent signal require the use of an added blocking step to decrease the signal generated by non-specific binding.
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