SCT043
BioTracker TP-HOCL1 Live Cell Dye
Live cell imaging dye for hypochlorus acid (HOCL) used to detect phagocytosis, inflammation and cellular apoptosis.
Synonym(s):
Live cell imaging probe
About This Item
Recommended Products
Assay
≥98% (H-NMR)
≥98% (HPLC)
≥98% (LC/MS)
≥98% (elemental analysis)
technique(s)
cell based assay: suitable
detection method
fluorometric
shipped in
ambient
1 of 4
This Item | AB5401 | MAB347 | SAB1405847 |
---|---|---|---|
Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
biological source rabbit | biological source rabbit | biological source mouse | biological source mouse |
Gene Information human ... GAP43(2596) | Gene Information human ... GAP43(2596) | Gene Information human ... GAP43(2596) | Gene Information human ... GAP43(2596) |
UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
clone polyclonal | clone polyclonal | clone 9-1E12, monoclonal | clone polyclonal |
General description
The BioTracker TP-HOCL1 dye is a live cell two-photon green fluorescent “turn-on” imaging probe for HOCL. The probe exhibit fast response times, good selectivity, and high sensitivity towards hypochlorous acid in living cells. Acedan was chosen as the fluorescence reporting group due to its excellent photophysical properties resulting from the typical “push−pull” (amineketone) structure. 2- mercaptoethanol and 1, 2-ethanedithiol were employed to protect the ketone of acedan in the design of HOCl probes. Reaction of the probe with HOCl, which deprotects the oxathiolane/mercaptal group to reveal the ketone, would lead to fluorescence enhancement
Spectral Properties
Absorbance: 375nm
Emission: 500nm
References
Chang YT et al. Development of Targetable Two-Photon Fluorescent Probes to Image Hypochlorous Acid in Mitochondria and Lysosome in Live Cell and Inflamed Mouse. Model. J Am Chem Soc. 2015 May 13;137(18):5930-8.
Application
Cell Imaging
Live Cell Dye
Quality
Molar Mass: 258.36 g/mol
Physical form
Storage and Stability
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Disclaimer
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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